Pentose Metabolism in <i>Clostridium acetobutylicum</i>

DOI Open Access
  • TOMOEDA Mikio
    Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University
  • HORITSU Hiroyuki
    Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University
  • SASAKI Ikuharu
    Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University

Bibliographic Information

Other Title
  • Pentose Metabolism in Clostridium acetobutylicum
  • Part I. L-Arabinose Ketol Isomerase

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Description

L-Arabinose ketol-isomerase (EC 5. 3. 1. 4) was extracted from the L-arabinose-grown cells of Clostridium acetobutylicum and partially purified by ammonium sulfate fractionation, DEAE-cellulose column chromatography and the gel filtration on Sephadex G-200.<br> Optimum pH and temperature for the activity of the enzyme were 7.5 and 37°C, respectively. The enzyme was inhibited by EDTA, Na-pyrophosphate and KCN, and its activity was restored by the addition of divalent metal ions such as Mn2+ and Co2+. The Michaelis-Menten's constants for L-arabinose, Mn2+ and Co2+ were 1.08×10-2M, 0.83×10-4M and 0.38×10-4M, respectively. The equilibrium constant (K=ribulose/arabinose) was 3.14 (37◊C, pH 7.5). The thermodynamic quantities, ΔH, ΔG (37°C), ΔS (37°C) and E were +7354cal/mole, -710.7cal/mole, +26.0cal/(deg•mole) and +7471cal/mole, respectively.

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Details 詳細情報について

  • CRID
    1390282681445207936
  • NII Article ID
    130004718899
  • DOI
    10.1271/bbb1961.33.151
  • COI
    1:CAS:528:DyaF1MXnsFalsQ%3D%3D
  • ISSN
    18811280
    00021369
  • Text Lang
    en
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
    • OpenAIRE
  • Abstract License Flag
    Disallowed

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