A Novel One-step Process for Enzymatic Incorporation of Amino Acids into Proteins : Papain-catalyzed Polymerization of<scp>l</scp>-Methionine Ethyl Ester and Its Regulation by Adding a Protein Substrate

DOI DOI オープンアクセス
  • ARAI Soichi
    Department of Agricultural Chemistry, University of Tokyo
  • YAMASHITA Michiko
    Department of Agricultural Chemistry, University of Tokyo
  • FUJIMAKI Masao
    Department of Agricultural Chemistry, University of Tokyo Department of Food and Nutrition, Ochanomizu University

書誌事項

タイトル別名
  • A novel one-step process for enzymatic incorporation of amino acids into proteins: Papain-catalyzed polymerization of L-methionine ethyl ester and its regulation by adding a protein substrate.

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説明

To enhance a methionine level of soy protein the plastein reaction was replaced by a simplified one-step process which had been newly developed as described in the preceding paper. It was found that during the process L-methionine ethyl ester (H•Met•OEt) underwent papain-catalyzed polymerization when the enzymatic incubation was carried out in the absence or inadequacy of soy protein. There was a critical point with regard to the ratio of the concen-tration of H•Met•OEt•HCl ([M]) and that of the soy protein ([S]). A sufficiently low [M]/[S] prevented H• Met•OEt from forming its polymer, permitting efficient incorporation of methio-nine into soy protein. A high possibility was suggested that in such a case H•Met•OEt acted as a nucleophile amine to be incorporated in the mode of papain-catalyzed aminolysis of protein.

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詳細情報 詳細情報について

  • CRID
    1390282681445310720
  • NII論文ID
    130003526462
  • DOI
    10.1271/bbb1961.43.1069
    10.1080/00021369.1979.10863579
  • COI
    1:CAS:528:DyaE1MXksFCrurk%3D
  • ISSN
    18811280
    00021369
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
    • OpenAIRE
  • 抄録ライセンスフラグ
    使用不可

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