Urocanic Acid Production by Sporogenous Bacteria

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  • IMANAKA Hiroshi
    Tokyo Research Laboratory of Fujisazva Pharmaceutical Industries, Ltd.

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  • Part II. Cultural Conditions and Mechanisms of Urocanic Acid Accumulation

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As the results of a screening of several type cultures of bacteria, Bacillus subtilis var. thermophilus was revealed to- be the most powerful strain for urocanic acid production. The accumulation of urocanic acid by this bacteria is caused by deamination of L-histidine, and is particularly accelerated in the presence of a component (X-factor) in meat extract. In the decomposition of urocanic acid the optimal pH of urocanase activity is markedly inhibited by the deviation of pH of the culture medium. The histidase of this bacteria is supposed to be a new exo-type enzyme.

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