Characterization of Molecular Structure of Starch Granules in Suspension-cultured Cells from<i>Ipomoea cordatotriloba</i>Denn.
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- KITAHARA Kanefumi
- Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University
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- IMAMURA Koki
- Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University
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- OMAE Yuko
- Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University
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- SUGANUMA Toshihiko
- Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University
書誌事項
- タイトル別名
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- Characterization of Molecular Structure of Starch Granules in Suspension-cultured Cells from Ipomoea cordatotriloba Denn.
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The molecular structure of starch granules formed in suspension-cultured cells of Ipomoea cordatotriloba Denn. was characterized by its chain length distribution, which was compared to those of the starches from the root and leaf of the original plant. The cultured cell starches were spherical and had a very small granule size (about 2 μm). The debranched starches roughly separated into three fractions during gel-permeation chromatography, and the fractions were defined as Fr.1, 2, and 3, respectively. The chain length distribution of the debranched cultured cell starch showed that the high molecular weight fraction (Fr.1), referred to as an amylose fraction, was much less than those of the root and leaf starches. The ratio of the two lower fractions (Fr.3/Fr.2) of the cultured cell starch, which was mainly derived from unit chains of amylopectin, was greatest among the starches, suggesting that the amylopectin from the cultured cell starch has much shorter unit chains. By X-ray diffraction analysis, it was found that both cultured cell and leaf starch granules have low crystallinity.<br>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 62 (10), 1962-1967, 1998
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681447298432
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- NII論文ID
- 110002679057
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DyaK1cXntlegtLw%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 4666565
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可