High Yield Synthesis of Nitriles by a New Enzyme, Phenylacetaldoxime Dehydratase, from Bacillus sp. Strain OxB-1
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- KATO Yasuo
- Biotechnology Research Center, Toyama Prefectural University
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- XIE Sheng-Xue
- Biotechnology Research Center, Toyama Prefectural University
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- ASANO Yasuhisa
- Biotechnology Research Center, Toyama Prefectural University
書誌事項
- タイトル別名
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- High Yield Synthesis of Nitriles by a New Enzyme, Phenylacetaldoxime Dehydratase, form Bacillus sp. Strain OxB-1.
- 公開日
- 2001
- 資源種別
- journal article
- DOI
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- 10.1271/bbb.65.2666
- 公開者
- 公益社団法人 日本農芸化学会
この論文をさがす
説明
3-Phenylpropionitrile was synthesized from Z-3-phenylpropionaldoxime (0.75 M) in a quantitative yield (98 g/l) by the use of cells of Eschrichia coli JM 109/pOxD-9OF, a transformant harboring a gene for a new enzyme, phenylacetaldoxime dehydratase, from Bacillus sp. strain OxB-1. Other arylalkyl- and alkyl-nitriles were also synthesized in high yields from the corresponding aldoximes. Moreover, 3-phenylpropionitrile was successfully synthesized by the recombinant cells in 70 and 100% yields from 0.1 M unpurified E/Z-3-phenylpropionaldoxime, which is spontaneously formed from 3-phenylpropionaldehyde and hydroxylamine in a butyl acetate/water biphasic system and aqueous phase, respectively.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 65 (12), 2666-2672, 2001
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681449574144
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- NII論文ID
- 110002693340
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD38XjtVygsA%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 6027298
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- PubMed
- 11826962
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- JaLC
- NDLサーチ
- Crossref
- PubMed
- CiNii Articles
- OpenAIRE
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- 抄録ライセンスフラグ
- 使用不可
