Production of New Restriction Endonuclease<i>Vpa</i>K111BI Recognizing Sequence 5′-GGWCC-3′ in a Haemolysin-less Mutant of<i>Vibrio parahaemolyticus</i>
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- Production of New Restriction Endonuclease VpaK11BI Recognizing Sequence 5'-GGWCC-3' in a Haemolysin-less Mutant of Vibrio parahaemolyticus.
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説明
High restriction endonuclease activity was found in a haemolysin-less mutant of the Vibrio parahaemolyticus 1743-1 strain. The endonuclease, named VpaK11BI, recognized the palindromic pentanucleotide sequence of 5'-GGWCC-3' and cleaved double-stranded DNA after the first G, which is exactly the same as the specificity of AvaII. The haemolysin-less mutant of V parahaemolyticus is now available for producing the valuable restriction endonuclease on a commercial scale.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 61 (12), 2129-2130, 1997
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681450456064
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- NII論文ID
- 110002678897
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- PubMed
- 27396891
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- 本文言語コード
- en
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- データソース種別
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- JaLC
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