Preparation of D-Gulose from Disaccharide Lactitol Using Microbial and Chemical Methods
-
- MORIMOTO Kenji
- Rare Sugar Research Center, Kagawa University
-
- SHIMONISHI Tsuyoshi
- Izumoring LLC
-
- MIYAKE Seiki
- Rare Sugar Research Center, Kagawa University
-
- TAKATA Goro
- Rare Sugar Research Center, Kagawa University
-
- IZUMORI Ken
- Izumoring LLC
書誌事項
- タイトル別名
-
- Preparation of <small>D</small>-Gulose from Disaccharide Lactitol Using Microbial and Chemical Methods
この論文をさがす
説明
When an M31 strain of Agrobacterium tumefaciens was grown in a mineral salt medium at 30 °C containing 1.0% lactitol as sole carbon source, a keto-sugar was efficiently accumulated in the supernatant. This oxidation from lactitol to the keto-sugar was caused by M31 cells grown with medium containing a disaccharide unit, including sucrose, lactitol, lactose, maltose, or maltitol, suggesting that the enzyme is inducible. M31 also demonstrated good growth characteristics in Tryptic Soy Broth (TSB) medium containing 1.0% sucrose, and cells grown under these conditions showed strong lactitol transformation activity. The keto-sugar product was reduced by chemical hydrogenation and the resulting product was hydrolyzed to D-gulose, D-galactose, and D-sorbitol by acid hydrolysis, revealing that the reduced products are lactitol and D-gulosyl-(β-1,4)-D-sorbitol. Taken together, these results indicate that M31 can convert lactitol to 3-ketolactitol and thus provide access to the rare sugar D-gulose.
収録刊行物
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 77 (2), 253-258, 2013
公益社団法人 日本農芸化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282681453601536
-
- NII論文ID
- 10031164724
-
- NII書誌ID
- AA10824164
-
- COI
- 1:STN:280:DC%2BC3szmsFKltQ%3D%3D
-
- ISSN
- 13476947
- 09168451
-
- NDL書誌ID
- 024295076
-
- PubMed
- 23391912
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可