High Shikimate Production from Quinate with Two Enzymatic Systems of Acetic Acid Bacteria
-
- ADACHI Osao
- Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
-
- ANO Yoshitaka
- Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
-
- TOYAMA Hirohide
- Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
-
- MATSUSHITA Kazunobu
- Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
この論文をさがす
抄録
3-Dehydroshikimate was formed with a yield of 57–77% from quinate via 3-dehydroquinate by two successive enzyme reactions, quinoprotein quinate dehydrogenase (QDH) and 3-dehydroquinate dehydratase, in the cytoplasmic membranes of acetic acid bacteria. 3-Dehydroshikimate was then reduced to shikimate (SKA) with NADP-dependent SKA dehydrogenase (SKDH) from the same organism. When SKDH was coupled with NADP-dependent D-glucose dehydrogenase (GDH) in the presence of excess D-glucose as an NADPH re-generating system, SKDH continued to produce SKA until 3-dehydroshikimate added initially in the reaction mixture was completely converted to SKA. Based on the data presented, a strategy for high SKA production was proposed.
収録刊行物
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 70 (10), 2579-2582, 2006
公益社団法人 日本農芸化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282681453735168
-
- NII論文ID
- 10018526303
-
- NII書誌ID
- AA10824164
-
- ISSN
- 13476947
- 09168451
-
- NDL書誌ID
- 8524736
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可