Enzymatic Production of γ-Aminobutyric Acid in Soybeans Using High Hydrostatic Pressure and Precursor Feeding
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- UENO Shigeaki
- Graduate School of Agricultural Science, Tohoku University
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- KATAYAMA Takumi
- Graduate School of Agricultural Science, Tohoku University
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- WATANABE Takae
- Department of Food Science, Niigata University of Pharmacy and Applied Life Sciences
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- NAKAJIMA Kanako
- Department of Food Science, Niigata University of Pharmacy and Applied Life Sciences
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- HAYASHI Mayumi
- Department of Food Science, Niigata University of Pharmacy and Applied Life Sciences
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- SHIGEMATSU Toru
- Department of Food Science, Niigata University of Pharmacy and Applied Life Sciences
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- FUJII Tomoyuki
- Graduate School of Agricultural Science, Tohoku University Department of Food Science, Niigata University of Pharmacy and Applied Life Sciences
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説明
The effects were investigated of the glutamic acid (Glu) substrate concentration on the generation and kinetics of γ-aminobutyric acid (GABA) in soybeans treated under high hydrostatic pressure (HHP; 200 MPa for 10 min at 25 °C). The conversion of Glu to GABA decreased with increasing initial Glu concentration in the soybeans. The crude glutamate decarboxylase (GAD) obtained from the HHP-treated soybeans showed substrate inhibition. The GABA production rate in the HHP-treated soybeans fitted the following substrate inhibition kinetic equation: v0=(VmaxS0)/(Km+S0+(S0)2/Ki). The Km value for the HHP-treated soybeans was significantly higher than that of the untreated soybeans. The Km values in this study show the affinity between Glu and GAD, and indicate that the HHP-treated soybeans had lower affinity between Glu and GAD than the untreated soybeans. GAD extracted from the HHP-treated soybeans showed a similar value to that in the HHP-treated soybeans. The intact biochemical system was so damaged in the HHP-treated soybeans that it showed substrate inhibition kinetics similar to that of the extracted GAD. The combination of HHP and precursor feeding proved to be a novel tool that can be used to increase the concentration of a target component.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 77 (4), 706-713, 2013
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681454230144
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- NII論文ID
- 10031170707
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- NII書誌ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC3srivFCgtA%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 024442327
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- PubMed
- 23563561
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可
