Characterization of recombinant β-amylases from Oryza sativa

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  • KOIDE Tomojiro
    Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo
  • OHNISHI Yasuo
    Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo
  • HORINOUCHI Sueharu
    Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo

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タイトル別名
  • Characterization of Recombinant .BETA.-Amylases from Oryza sativa
  • Characterization of recombinant v amylases from Oryza sativa
  • Characterization of Recombinant β-Amylases from<i>Oryza sativa</i>

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Four putative β-amylase genes found in the Oryza sativa cDNA sequence database (KOME) were expressed in Escherichia coli. Recombinant proteins from two of these genes showed β-amylase activity. Similarly to β-amylases from other plants, the optimum pH of the recombinant rice β-amylases was about 5.5–6.0, but they exhibited inferior heat stability to soybean β-amylase.

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