Gene Cloning and Biochemical Characterization of the BMP-2 of Pinctada fucata

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  • MIYASHITA Tomoyuki
    Department of Genetic Engineering, Faculty of Biology-Oriented Science and Technology, Kinki University
  • HANASHITA Takuo
    Department of Genetic Engineering, Faculty of Biology-Oriented Science and Technology, Kinki University
  • TORIYAMA Michinori
    Department of Genetic Engineering, Faculty of Biology-Oriented Science and Technology, Kinki University
  • TAKAGI Ryousuke
    Department of Genetic Engineering, Faculty of Biology-Oriented Science and Technology, Kinki University
  • AKASHIKA Tamaki
    Department of Genetic Engineering, Faculty of Biology-Oriented Science and Technology, Kinki University
  • HIGASHIKUBO Nobuto
    Department of Genetic Engineering, Faculty of Biology-Oriented Science and Technology, Kinki University

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  • Gene Cloning and Biochemical Characterization of the BMP-2 of<i>Pinctada fucata</i>

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Abstract

The bone morphogenetic proteins (BMPs) constitute a subfamily of the transforming growth factor type beta (TGF-β) supergene family. BMP-2 plays an important role not only in osteoblast differentiation but also in pattern formation during development. To determine the function of BMP-2 in Pinctada fucata development and hard tissue formation, we isolated a BMP-2 genomic DNA clone and the BMP-2 cDNA. The deduced BMP-2 sequence consisted of 447 amino acids. The BMP-2 gene was composed of three exons. The C-terminal portion (149 amino acids) had 86% and 66% identity to the Crassostrea gigas and the human BMP-2 sequence respectively. The 5′ flanking promoter region contained putative glioma transcription factor (Gli) and retinoic acid receptor (RAR) responding elements. The BMP-2 gene was expressed strongly in the inner part of the mantle tissue, corresponding to the nacreous aragonite shell layer. This finding suggests that BMP-2 has a key role in nacreous layer formation.

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