Gene Cloning and Biochemical Characterization of the BMP-2 of<i>Pinctada fucata</i>
-
- MIYASHITA Tomoyuki
- Department of Genetic Engineering, Faculty of Biology-Oriented Science and Technology, Kinki University
-
- HANASHITA Takuo
- Department of Genetic Engineering, Faculty of Biology-Oriented Science and Technology, Kinki University
-
- TORIYAMA Michinori
- Department of Genetic Engineering, Faculty of Biology-Oriented Science and Technology, Kinki University
-
- TAKAGI Ryousuke
- Department of Genetic Engineering, Faculty of Biology-Oriented Science and Technology, Kinki University
-
- AKASHIKA Tamaki
- Department of Genetic Engineering, Faculty of Biology-Oriented Science and Technology, Kinki University
-
- HIGASHIKUBO Nobuto
- Department of Genetic Engineering, Faculty of Biology-Oriented Science and Technology, Kinki University
書誌事項
- タイトル別名
-
- Gene Cloning and Biochemical Characterization of the BMP-2 of Pinctada fucata
この論文をさがす
抄録
The bone morphogenetic proteins (BMPs) constitute a subfamily of the transforming growth factor type beta (TGF-β) supergene family. BMP-2 plays an important role not only in osteoblast differentiation but also in pattern formation during development. To determine the function of BMP-2 in Pinctada fucata development and hard tissue formation, we isolated a BMP-2 genomic DNA clone and the BMP-2 cDNA. The deduced BMP-2 sequence consisted of 447 amino acids. The BMP-2 gene was composed of three exons. The C-terminal portion (149 amino acids) had 86% and 66% identity to the Crassostrea gigas and the human BMP-2 sequence respectively. The 5′ flanking promoter region contained putative glioma transcription factor (Gli) and retinoic acid receptor (RAR) responding elements. The BMP-2 gene was expressed strongly in the inner part of the mantle tissue, corresponding to the nacreous aragonite shell layer. This finding suggests that BMP-2 has a key role in nacreous layer formation.
収録刊行物
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 72 (1), 37-47, 2008
公益社団法人 日本農芸化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282681454826880
-
- NII論文ID
- 10027522787
-
- NII書誌ID
- AA10824164
-
- ISSN
- 13476947
- 09168451
-
- NDL書誌ID
- 9364941
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可