Enhancement of Thermal Stabilization of Formaldehyde Dehydrogenase from<i>Pseudomonas putida</i>by Directed Evolution
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- IMAMURA Chie
- Toyota Central R&D Labs., Inc.
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- SHIGEMORI Yasushi
- Aisin Cosmos R&D Co., Ltd.
書誌事項
- タイトル別名
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- Enhancement of Thermal Stabilization of Formaldehyde Dehydrogenase from Pseudomonas putida by Directed Evolution
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We used directed evolution to enhance the thermostability of formaldehyde dehydrogenase from Pseudomonas putida. At 50 °C, the wild-type enzyme was inactivated within 30 min, but the variants obtained retained 80% activity for at least 300 min. At room temperature (30 °C), the variants obtained retained <80% activity for at least 500 h (21 d).
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 74 (7), 1462-1465, 2010
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681456231680
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- NII論文ID
- 10027557477
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 10766732
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
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