Archaeal Aldehyde Dehydrogenase ST0064 from Sulfolobus tokodaii, a Paralog of Non-Phosphorylating Glyceraldehyde-3-phosphate Dehydrogenase, Is a Succinate Semialdehyde Dehydrogenase
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- ITO Fumiaki
- Department of Biotechnology, The University of Tokyo
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- CHISHIKI Hidehiro
- Department of Biotechnology, The University of Tokyo
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- FUSHINOBU Shinya
- Department of Biotechnology, The University of Tokyo
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- WAKAGI Takayoshi
- Department of Biotechnology, The University of Tokyo
書誌事項
- タイトル別名
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- Archaeal Aldehyde Dehydrogenase ST0064 from <i>Sulfolobus tokodaii</i>, a Paralog of Non-Phosphorylating Glyceraldehyde-3-phosphate Dehydrogenase, Is a Succinate Semialdehyde Dehydrogenase
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Aldehyde dehydrogenase ST0064, the closest paralog of previously characterized allosteric non-phosphorylating glyceraldehyde-3-phosphate (GAP) dehydrogenase (GAPN, ST2477) from a thermoacidophilic archaeon, Sulfolobus tokodaii, was expressed heterologously and characterized in detail. ST0064 showed remarkable activity toward succinate semialdehyde (SSA) (Km of 0.0029 mM and kcat of 30.0 s−1) with no allosteric regulation. Activity toward GAP was lower (Km of 4.6 mM and kcat of 4.77 s−1), and previously predicted succinyl-CoA reductase activity was not detected, suggesting that the enzyme functions practically as succinate semialdehyde dehydrogenase (SSADH). Phylogenetic analysis indicated that archaeal SSADHs and GAPNs are closely related within the aldehyde dehydrogenase superfamily, suggesting that they are of the same origin.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 77 (6), 1344-1348, 2013
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681456478592
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- NII論文ID
- 10031184711
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- NII書誌ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC3sjhtFWntw%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 024647337
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- PubMed
- 23748791
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可