Overproduction of Hyperthermostable β-1,4-Endoglucanase from the Archaeon Pyrococcus horikoshii by Tobacco Chloroplast Engineering
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- NAKAHIRA Yoichi
- Venture Business Laboratory, Ehime University Graduate School of Life and Environmental Sciences Kyoto Prefectural University Phytoculture Control Co., Ltd.
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- ISHIKAWA Kazuhiko
- Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology (AIST)
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- TANAKA Kunisuke
- Graduate School of Life and Environmental Sciences Kyoto Prefectural University Phytoculture Control Co., Ltd.
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- TOZAWA Yuzuru
- Venture Business Laboratory, Ehime University Proteo-Science Center, Ehime University
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- SHIINA Takashi
- Graduate School of Life and Environmental Sciences Kyoto Prefectural University
書誌事項
- タイトル別名
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- Overproduction of Hyperthermostable β-1,4-Endoglucanase from the Archaeon <i>Pyrococcus horikoshii</i> by Tobacco Chloroplast Engineering
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One of the most cost-effective methods of producing industrial enzymes is by the use of transgenic plants. We demonstrated successful high-level expression of a hyperthermostable archaeal β-1,4-endoglucanase in mature tobacco leaves by transformation of chloroplasts by homologous recombination. The active recombinant enzyme was readily recovered not only from fresh but also from dried leaves.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 77 (10), 2140-2143, 2013
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681456859392
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- NII論文ID
- 130003381889
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- NII書誌ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC2c%2FjtFOrtQ%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 024972369
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- PubMed
- 24096651
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可