Effects of Cellulose Crystallinity, Hemicellulose, and Lignin on the Enzymatic Hydrolysis of<i>Miscanthus sinensis</i>to Monosaccharides

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  • YOSHIDA Makoto
    Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • LIU Yuan
    Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • UCHIDA Satoshi
    Tokyo Metropolitan Industrial Technology Research Institute
  • KAWARADA Kensuke
    Tokyo Metropolitan Industrial Technology Research Institute
  • UKAGAMI Yusuke
    Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • ICHINOSE Hitomi
    Food Biotechnology Division, National Food Research Institute
  • KANEKO Satoshi
    Food Biotechnology Division, National Food Research Institute
  • FUKUDA Kiyoharu
    Faculty of Agriculture, Tokyo University of Agriculture and Technology

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タイトル別名
  • Effects of Cellulose Crystallinity, Hemicellulose, and Lignin on the Enzymatic Hydrolysis of Miscanthus sinensis to Monosaccharides
  • Effects of cellulose crystallinity, hemisellulose, and lignin on the enzymatic hydrolysis of Miscanthus sinensis to monosaccharides
  • Effect of cellulose crystallinity, hemicelluloses, and lignin on enzymatic hydrolysis of Miscanthus sinesis to monosaccharides

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説明

The effects of cellulose crystallinity, hemicellulose, and lignin on the enzymatic hydrolysis of Miscanthus sinensis to monosaccharides were investigated. A air-dried biomass was ground by ball-milling, and the powder was separated into four fractions by passage through a series of sieves with mesh sizes 250–355 μm, 150–250 μm, 63–150 μm, and <63 μm. Each fraction was hydrolyzed with commercially available cellulase and β-glucosidase. The yield of monosaccharides increased as the crystallinity of the substrate decreased. The addition of xylanase increased the yield of both pentoses and glucose. Delignification by the sodium chlorite method improved the initial rate of hydrolysis by cellulolytic enzymes significantly, resulting in a higher yield of monosaccharides as compared with that for untreated samples. When delignified M. sinensis was hydrolyzed with cellulase, β-glucosidase, and xylanase, hemicellulose was hydrolyzed completely into monosaccharides, and the conversion rate of glucan to glucose was 90.6%.

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