Sample Preparation Methods for Large-Scale Analysis of Membrane Proteins Using Shotgun Proteomics

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  • MASUDA Takeshi
    Institute for Advanced Biosciences, Keio University
  • ISHIHAMA Yasushi
    Institute for Advanced Biosciences, Keio University PRESTO, Japan Science and Technology Agency

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  • ショットガンプロテオミクスによる膜タンパク質の網羅的解析のための試料調製法
  • ショットガンプロテオミクス ニ ヨル マク タンパクシツ ノ モウラテキ カイセキ ノ タメ ノ シリョウ チョウセイホウ

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Shotgun proteomics based on liquid chromatography/mass spectrometry has been widely used to provide a large-scale view of the proteome, including protein expression, localization, modification, and protein-protein interaction. However, it is still challenging tasks to carry out membrane protein-targeted proteome analysis due to the hydrophobic nature of proteins with transmembrane domains. Here, we review current sample preparation techniques such as enrichment, solubilization, and digestion for membrane proteomics and focus on phase-transfer surfactant-based approaches recently developed by our group. We also discuss future developments for membrane proteome focused shotgun proteomics.

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