バイセルに結合したペプチドの高分解能｀１´Ｈ　ＮＭＲ観測

DOI

書誌事項

タイトル別名
  • Direct Observations of High Resolution 1H NMR in Liquid Phase for Peptides bound to Bicelles

この論文をさがす

説明

Direct observations of high resolution 1H NMR in liquid phase for Valinomycin bound to bicelles as a model of membrane bound peptides were endeavored using a high resolution magic angle spinning (HR-MAS) probe. The bicelles were composed of two kinds of lipids, dimyristoyl phosphatidylcholine (DMPC) and 3- [ (3-cholamidopropyl) dimethylanunonio] -2-hydroxypropane sulfonic acid (CHAPSO). The equivalent amounts of these lipids were mixed. Using both a HR-MAS probe and bicelles, we observed high resolution 1H NMR spectra of the peptide directly and successfully. Amide protons of valinomycin bound to bicelles were observed even in deuterium oxide solution and after sonication. Such results suggest that valinomycin forms intra molecular hydrogen bonds and is bound to bicelles. Assignments of the amide protons of valinomycin bound to bicelles were performed from the multiplicity and NOESY spectrum using a HR-MAS probe.

収録刊行物

  • 高分子論文集

    高分子論文集 60 (4), 199-202, 2003

    公益社団法人 高分子学会

キーワード

詳細情報 詳細情報について

  • CRID
    1390282681498292608
  • NII論文ID
    130004035601
  • DOI
    10.1295/koron.60.199
  • ISSN
    18815685
    03862186
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

問題の指摘

ページトップへ