書誌事項
- タイトル別名
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- Effect of RNAi-induced inhibition of ets-1 gene expression on invasion properties of oral squamous carcinoma cells-Functional evaluation of ets-1 gene-
- コウクウ ヘンペイ ジョウヒガン サイボウ ノ シンジュンノウ ニ オヨボス RNAi オ モチイタ ets 1 イデンシ ハツゲン ヨクセイ ノ エイキョウ ets 1 イデンシ ノ キノウ ヒョウカ
- Functional evaluation of<I>ets</I>-1 gene
- <I>ets</I>-1遺伝子の機能評価
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抄録
Metastasis in invasive carcinoma is closely associated with the production of extracellular matrix catabolic enzymes, such as matrix metalloproteinases (MMPs). It is known thatetsproto-oncogenes exist in transcriptional regulation refions of the MMP gene and enhance or inhibit MMP transcription. In this study, we restrained theets-1 gene expression of an oral squamous carcinoma cell line by means of RNA interference (RNAi) and examined the change in invasion ability from a molecular biological perspective. An oral squamous carcinoma cell line (HSC-3) was transfected with short interfering RNA (siRNA) ofets-1. The invasive ability of the transfected cells was determined by a matrigel invasion assay, and MMP-9 production was measured by gelatin zymography. These data were compared with those of normal cultured cells. Matrigel invasion assay demonstrated that the transfected cells had a lower invasive ability than HSC-3, and gelatin zymography demonstrated that the MMP-9 production in these cells had decreased. These results suggested that transfection of siRNAets-1 would inhibit tumor invasion through down-regulation of MMP-9.
収録刊行物
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- 日本口腔外科学会雑誌
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日本口腔外科学会雑誌 53 (1), 2-7, 2007
社団法人 日本口腔外科学会
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詳細情報 詳細情報について
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- CRID
- 1390282681508583168
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- NII論文ID
- 10018858475
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- NII書誌ID
- AN00189163
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- ISSN
- 21861579
- 00215163
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- NDL書誌ID
- 8659025
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可