The Development of a Novel Diagnostic Assay That Utilizes a Pseudotyped Vesicular Stomatitis Virus for the Detection of Neutralizing Activity against Crimean-Congo Hemorrhagic Fever Virus

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  • Suda Yuto
    Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo Special Pathogens Laboratory, Department of Virology I, National Institute of Infectious Diseases
  • Chamberlain John
    National Infection Service, Public Health England
  • Dowall Stuart D.
    National Infection Service, Public Health England
  • Saijo Masayuki
    Special Pathogens Laboratory, Department of Virology I, National Institute of Infectious Diseases
  • Horimoto Taisuke
    Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Hewson Roger
    National Infection Service, Public Health England
  • Shimojima Masayuki
    Special Pathogens Laboratory, Department of Virology I, National Institute of Infectious Diseases

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<p>Crimean-Congo hemorrhagic fever virus is a risk group 4 pathogen, which mandates the use of maximum containment facilities, often termed biosafety level 4 or containment level 4 when working with infectious materials. Diagnostic and research work involving live viruses in such laboratories is time-consuming and inconvenient, resulting in delays. Herein, we show that serum neutralizing activity against the virus can be measured in low-containment laboratories using a pseudotyped virus.</p>

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