Stability of Serum Lactate Dehydrogenase Activity and Isozyme Pattern in Cattle Based on Storage Time and Temperature

DOI JASI Web Site Web Site 1 Citations 4 References
  • YOSHIDA Yuichi
    Hokubu Agricultural Technology Institute, Hyogo Prefectural Technology Center for Agriculture, Forest and Fishery
  • NAKAMURA Makoto
    Kagoshima Central Livestock Hygiene Service Center
  • SAWADA Hiroshi
    National Institute of Animal Health, National Agricultural and Food Research Organization
  • ARAI Shozo
    National Institute of Animal Health, National Agricultural and Food Research Organization

Bibliographic Information

Other Title
  • 牛血清の保存温度と保存期間が乳酸脱水素酵素の活性及びアイソザイムに及ぼす影響
  • ギュウ ケッセイ ノ ホゾン オンド ト ホゾン キカン ガ ニュウサン ダッスイソ コウソ ノ カッセイ オヨビ アイソザイム ニ オヨボス エイキョウ

Search this article

Abstract

<p>This study aimed to assess the stability of serum lactate dehydrogenase (LDH) activity and isozyme pattern based on storage time and temperature in cattle. Serum from Holstein and Japanese black cattle was stored at a variety of temperatures (4, −20 and −80 ℃). We also examined the serum of swine (Yorkshire) as a control. In Holstein and Japanese black cattle, there were no significant differences in serum LDH activity and isozyme pattern for 12 months at −20 and −80 ℃. On the other hand, LDH activity of stored serum at −20 ℃ in swine decreased significantly (P＜0.05) after 1 month. The M subunit ratio of LDH in swine serum was high compared with bovine serum. It was suggested that LDH activity and isozyme pattern using the bovine serum stored at −20 ℃ was stabilized for 12 months.</p>

Journal

Citations (1)*help

See more

References(4)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top