Generation of specific aptamers targeting MRGPRX2using synthetic proteoliposomes

DOI Web Site Open Access

Bibliographic Information

Other Title
  • MRGPRX2に対するアプタマー製剤の開発

Description

<p>Mas-related G protein coupled receptor-X2 (MRGPRX2) is a novel G protein-coupledreceptor with unique features including selective expression and response topeptidergic drug-induced activation in mast cells. With the aim of inhibitingMRGPRX2-dependent mast cell activation, a specific functional aptamer wasgenerated using the proteoliposome-based systematic evolution of ligands byexponential enrichment. After multiple cycles of selection and evolution,potential functional aptamers with high affinity and specificity were selectedby binding assay and functional screening and enriched for sequencing. Severalaptamers showed dose-dependent inhibitory potency on MRGPRX-dependent mast cellactivation. The feasibility and efficacy of these aptamers on drug-inducedanaphylactoid reactions will be investigated in our future planned study.</p>

Journal

Keywords

Details 詳細情報について

Report a problem

Back to top