MRGPRX2に対するアプタマー製剤の開発

DOI Web Site オープンアクセス

書誌事項

タイトル別名
  • Generation of specific aptamers targeting MRGPRX2using synthetic proteoliposomes

説明

<p>Mas-related G protein coupled receptor-X2 (MRGPRX2) is a novel G protein-coupledreceptor with unique features including selective expression and response topeptidergic drug-induced activation in mast cells. With the aim of inhibitingMRGPRX2-dependent mast cell activation, a specific functional aptamer wasgenerated using the proteoliposome-based systematic evolution of ligands byexponential enrichment. After multiple cycles of selection and evolution,potential functional aptamers with high affinity and specificity were selectedby binding assay and functional screening and enriched for sequencing. Severalaptamers showed dose-dependent inhibitory potency on MRGPRX-dependent mast cellactivation. The feasibility and efficacy of these aptamers on drug-inducedanaphylactoid reactions will be investigated in our future planned study.</p>

収録刊行物

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ