Effect of D.C. High Electric Field Application on Sterilization of <i>Escherichia coli </i>in Ice

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  • SATO Takunao
    Division of Electrical and Electronic Engineering, Graduate School of Science and Technology,The Meijo University
  • MURAKAMI Yuichi
    Department of Electrical and Electronic Engineering, Faculty of Science and Technology,The Meijo University
  • MURAMOTO Yuji
    Department of Electrical and Electronic Engineering, Faculty of Science and Technology,The Meijo University

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Other Title
  • 氷中の大腸菌殺菌に及ぼす直流高電界印加の効果
  • コオリ チュウ ノ ダイチョウキン サッキン ニ オヨボス チョクリュウ コウデンカイ インカ ノ コウカ

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<p>This paper reports the effect of D. C. high electric field application on sterilization of ice. The sample was Escherichia coli (JM103) in 1×10-3 mol/L or 5×10-3 mol/L NaCl aqueous solution. The experimental sample placed in a cuvette with parallel electrode plates (the distance between the electrodes was 1 mm), cooled at -20℃ for 20min. After freeging, D.C. voltage 500V were applied to the sample at -20℃. The survival ratio of E. coli in 1×10-3 mol/L was decreased under freezing injury. The survival ratio of E. coli in 5×10-3 mol/L was decreased under freezing injury and D.C. voltage application. In 1×10-3 mol/L or 5×10-3 mol/L NaCl aqueous solution, we could not be confirmed that effect of application time of D.C voltage on the survival ratio of E. coli.</p>

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