Section SEM and Serial Section SEM
-
- Kusumi Satoshi
- 鹿児島大学大学院医歯学総合研究科形態科学分野
-
- Koga Daisuke
- 旭川医科大学解剖学講座顕微解剖学分野
-
- Shibata Masahiro
- 鹿児島大学大学院医歯学総合研究科形態科学分野
-
- Watanabe Tsuyoshi
- 旭川医科大学解剖学講座顕微解剖学分野
Bibliographic Information
- Other Title
-
- 切片SEM法と連続切片SEM法
- セッペン SEMホウ ト レンゾク セッペン SEMホウ
Search this article
Abstract
<p>Section scanning electron microscopy (SEM) is a novel SEM technique based on observation of backscattered electron (BSE) images of tissue sections embedded in resin on glass microscope slides. Using this technique, high-resolution transmission electron microscopy (TEM)-like images can be obtained without the need to use specialized techniques such as ultramicrotomy. In this paper, we introduce three techniques based on the SEM method: 1) BSE imaging of semithin sections; 2) correlation of immunofluorescence and BSE images of the semithin sections; and 3) combination of a cryosectioning method (the Tokuyasu method) with section SEM. Additionally, we describe serial section SEM, which is a novel 3D imaging method, and discuss the possibilities for use of these SEM imaging techniques for tissue sections.</p>
Journal
-
- KENBIKYO
-
KENBIKYO 55 (1), 18-22, 2020-04-30
The Japanese Society of Microscopy
- Tweet
Keywords
Details
-
- CRID
- 1390285300156432128
-
- NII Article ID
- 130007840822
-
- NII Book ID
- AA11917781
-
- ISSN
- 24342386
- 13490958
-
- NDL BIB ID
- 030455600
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
- NDL
- CiNii Articles
- KAKEN
-
- Abstract License Flag
- Disallowed