Relationship between red blood cell aging and intracellular chemokine storage

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  • Ogasawara Shu
    Clinical Laboratory, Hirosaki University Hospital Department of Clinical Laboratory Medicine, Hirosaki University Graduate School of Medicine
  • Saito Norihiro
    Clinical Laboratory, Hirosaki University Hospital Department of Clinical Laboratory Medicine, Hirosaki University Graduate School of Medicine
  • Minakawa Satoko
    Clinical Laboratory, Hirosaki University Hospital
  • Akasaki Tomomi
    Clinical Laboratory, Hirosaki University Hospital
  • Kayaba Hiroyuki
    Clinical Laboratory, Hirosaki University Hospital Department of Clinical Laboratory Medicine, Hirosaki University Graduate School of Medicine

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Background: The Duffy antigen receptor for chemokines （DARC） is expressed on RBCs and is a non-signaling receptor for multiple chemokines. In the previous study, we had shown that DARC-affinity chemokines are stored in the RBCs. However, the fate of intracellularly stored chemokines has yet to be investigated. This study investigated whether RBCs retain the ability to accumulate these chemokines as they age.<br> Methods: Peripheral blood from five healthy volunteers were collected, and then transferred to hematocrit capillary tubes. After centrifugation, the RBC layer was divided to compare the top, upper, middle and bottom fractions. HbA1c, eotaxin-1 and RANTES were measured in each fraction.<br> Results: HbA1c increased significantly from the top fraction to the bottom fraction. Eotaxin-1 decreased significantly from the top fraction to the bottom fraction. RANTES exhibited a similar decrease, although no significant differences were observed. Eotaxin-1 showed a negative correlation（ r = -0.51, p = 0.03） with HbA1c. Although not statistically significant, RANTES showed a decreasing tendency （r = -0.40, p = 0.08） with the increase of HbA1c levels. These results indicate that RANTES and eotaxin-1 decrease rather than increase with RBC aging.<br> Conclusion: Eotaxin-1 and RANTES stored in RBCs tended to decrease as RBCs age.

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  • 弘前医学

    弘前医学 71 (2-4), 113-119, 2021

    弘前大学大学院医学研究科・弘前医学会

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