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- Hanaoka Nozomu
- Center for Infectious Disease Risk Management, National Institute of Infectious Diseases Private Care Clinic Tokyo
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- Nakayama Shuichi
- Department of Bacteriology I, National Institute of Infectious Diseases
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- Onoe Yasuhiko
- Private Care Clinic Tokyo
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- Nojiri Naomi
- Center for Infectious Disease Risk Management, National Institute of Infectious Diseases
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- Manda Kazushi
- Private Care Clinic Tokyo
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- Ohnishi Makoto
- Department of Bacteriology I, National Institute of Infectious Diseases
Bibliographic Information
- Other Title
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- 梅毒核酸検査における唾液やうがい液の有用性についての検討
- バイドク カクサン ケンサ ニ オケル ダエキ ヤ ウ ガ イ エキ ノ ユウヨウセイ ニ ツイテ ノ ケントウ
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Description
Recently, the number of syphilis cases has increased in Japan. The collection of blood and abrasion samples is invasive. Determination of the syphilis pathogen Treponema pallidum (TP) from patient specimens is critical because it provides a definitive diagnosis. Therefore, in this study, we have evaluated the usefulness of the nucleic acid test for TP using saliva and gargle fluids, which are easy to collect. Saliva and gargle specimens from patients who tested positive (74 patients) and negative (4 patients) for syphilis by various antibody tests were used. Two different nucleic acid testing methods targeting each of the 2 TP genes were used. The sensitivities of polymerase chain reaction (PCR) and real-time PCR were 21.6% and 36.5%, respectively. The specificity was 100% for both methods. Sensitivity depended on the duration of syphilis and gender. Especially in females in the secondary stage of syphilis, the sensitivity of real-time PCR was 100%.A certain number of TP nucleic acids were detected from saliva or gargle specimens; therefore, the development of a simple, minimally invasive, and QOL-improved screening method is expected in the future.
Journal
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- 日本性感染症学会誌
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日本性感染症学会誌 31 (1), 107-112, 2020-12-31
Japanese Society for Sexually Transmitted Infections
