Plant Regeneration by Tissue Culture from Immature Seeds of <i>Magnolia sieboldii</i> subsp. <i>japonica</i>

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  • Mori Junya
    Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • Tsukada Kentaro
    Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • Inoue Ryohei
    Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • Nozawa Yoko
    Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • Yamagishi Yusuke
    Research Faculty of Agriculture, Hokkaido University
  • Yasue Koh
    Faculty of Agriculture, Shinshu University Mountain Research Institute, Shinshu University
  • Nakaba Satoshi
    Faculty of Agriculture, Tokyo University of Agriculture and Technology Institute of Global Innovation Research, Tokyo University of Agriculture and Technology
  • Funada Ryo
    Faculty of Agriculture, Tokyo University of Agriculture and Technology Institute of Global Innovation Research, Tokyo University of Agriculture and Technology

Bibliographic Information

Other Title
  • オオヤマレンゲの未成熟種子を用いた組織培養による植物体再生に関する研究
  • オオヤマレンゲ ノ ミセイジュク シュシ オ モチイタ ソシキ バイヨウ ニ ヨル ショクブツタイ サイセイ ニ カンスル ケンキュウ

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Description

<p>Magnolia sieboldii subsp. japonica (Oyama renge) is a deciduous tree that is native to Japan. This tree has value as a rarity because it is designated as an endangered species in some prefectures and, therefore, its conservation and reproduction are very important. We initially cultured immature seeds on Lepoivre medium (LP), half of nitrogen source of Murashige and Skoog medium (1/2N MS) and Woody Plant Medium (WPM) containing α-naphthaleneacetic acid (NAA) as an auxin and 6-benzylaminopurine (BAP) as a cytokinin. Proembryogenic masses (PEMs) were induced directly from immature seeds and somatic embryos were induced from PEMs. Immature seeds one to three weeks after flowering might be suitable to induce PEMs. Some somatic embryos formed roots and hypocotyls under condition of lighting. Finally, we transferred the somatic embryo-derived plantlets to soil in pots and the plantlets continued to grow. Thus, we developed a tissue culture system for plant regeneration for the propagation of M. sieboldii subsp. japonica.</p>

Journal

  • Mokuzai Gakkaishi

    Mokuzai Gakkaishi 68 (4), 145-153, 2022-10-25

    The Japan Wood Research Society

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