Survival of anaerobic fungus Caecomyces sp. in various preservation methods : a comparative study
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- Nagpal Ravinder
- Dairy Microbiology Division, National Dairy Research Institute, Karnal 132001, Haryana, India
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- Puniya Anil Kumar
- Dairy Microbiology Division, National Dairy Research Institute, Karnal 132001, Haryana, India
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- Sehgal Jatinder Paul
- Dairy Cattle Nutrition Division, National Dairy Research Institute, Karnal 132001, Haryana, India
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- Singh Kishan
- Dairy Microbiology Division, National Dairy Research Institute, Karnal 132001, Haryana, India
書誌事項
- タイトル別名
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- Survival of anaerobic fungus <i>Caecomyces</i> sp. in various preservation methods: a comparative study
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説明
<p>The present investigation was designed to observe the survival of the anaerobic fungus Caecomyces sp. in various routine preservation methods. Among all the treatments, cryopreservation of fungi at −70°C with glycerol was found to be most effective for long-term maintenance (more than 90 days) of rumen fungi, followed by dimethyl sulfoxide (DMSO) and ethylene glycol (up to 60 days). In contrast, at −196°C, DMSO showed maximum survival (more than 90 days), followed by glycerol (up to 90 days) and ethylene glycol (up to 30 days). At 39°C, maximum survival (up to 30 days) was observed with soft agar and wheat straw; at refrigeration temperature, preservation with Orpin's media containing straw showed maximum survival (up to 30 days).</p>
収録刊行物
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- Mycoscience
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Mycoscience 53 (6), 427-432, 2012
一般社団法人 日本菌学会
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詳細情報 詳細情報について
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- CRID
- 1390295603315655936
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- NII論文ID
- 10031127273
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- NII書誌ID
- AA11003436
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- ISSN
- 16182545
- 13403540
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- NDL書誌ID
- 024102956
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用可