Conditional kisspeptin neuron-specific <i>Kiss1</i> knockout with newly generated <i>Kiss1</i>-floxed and <i>Kiss1</i>-Cre mice replicates a hypogonadal phenotype of global <i>Kiss1</i> knockout mice
-
- IKEGAMI Kana
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
-
- GOTO Teppei
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki 444-8585, Japan
-
- NAKAMURA Sho
- Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
-
- WATANABE Youki
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
-
- SUGIMOTO Arisa
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
-
- MAJARUNE Sutisa
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
-
- HORIHATA Kei
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
-
- NAGAE Mayuko
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
-
- TOMIKAWA Junko
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
-
- IMAMURA Takuya
- Graduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima 739-8526, Japan
-
- SANBO Makoto
- Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki 444-8585, Japan
-
- HIRABAYASHI Masumi
- Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki 444-8585, Japan
-
- INOUE Naoko
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
-
- MAEDA Kei-ichiro
- Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
-
- TSUKAMURA Hiroko
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
-
- UENOYAMA Yoshihisa
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
Bibliographic Information
- Other Title
-
- Conditional kisspeptin neuron-specific Kiss1 knockout with newly generated Kiss1-floxed and Kiss1-Cre mice replicates a hypogonadal phenotype of global Kiss1 knockout mice
Search this article
Description
<p> The present study aimed to evaluate whether novel conditional kisspeptin neuron-specific Kiss1 knockout (KO) mice utilizing the Cre-loxP system could recapitulate the infertility of global Kiss1 KO models, thereby providing further evidence for the fundamental role of hypothalamic kisspeptin neurons in regulating mammalian reproduction. We generated Kiss1-floxed mice and hypothalamic kisspeptin neuron-specific Cre-expressing transgenic mice and then crossed these two lines. The conditional Kiss1 KO mice showed pubertal failure along with a suppression of gonadotropin secretion and ovarian atrophy. These results indicate that newly-created hypothalamic Kiss1 KO mice obtained by the Cre-loxP system recapitulated the infertility of global Kiss1 KO models, suggesting that hypothalamic kisspeptin, but not peripheral kisspeptin, is critical for reproduction. Importantly, these Kiss1-floxed mice are now available and will be a valuable tool for detailed analyses of roles of each population of kisspeptin neurons in the brain and peripheral kisspeptin-producing cells by the spatiotemporal-specific manipulation of Cre expression.</p>
Journal
-
- Journal of Reproduction and Development
-
Journal of Reproduction and Development 66 (4), 359-367, 2020
The Society for Reproduction and Development
- Tweet
Details 詳細情報について
-
- CRID
- 1390566775159848448
-
- NII Article ID
- 130007888736
-
- NII Book ID
- AA10936678
-
- ISSN
- 13484400
- 09168818
-
- NDL BIB ID
- 030626395
-
- PubMed
- 32307336
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
-
- Abstract License Flag
- Disallowed