Exploration of <i>Salmonella</i> effector mutant strains on MTR4 and RRP6 degradation
-
- Sun Xiaoning
- Isotope Science Center, The University of Tokyo, Tokyo, Japan. Advanced Interdisciplinary Studies, Engineering Department, The University of Tokyo, Tokyo, Japan.
-
- Kawata Kentaro
- Isotope Science Center, The University of Tokyo, Tokyo, Japan.
-
- Miki Atsuko
- Isotope Science Center, The University of Tokyo, Tokyo, Japan.
-
- Wada Youichiro
- Isotope Science Center, The University of Tokyo, Tokyo, Japan. Advanced Interdisciplinary Studies, Engineering Department, The University of Tokyo, Tokyo, Japan.
-
- Nagahama Masami
- Laboratory of Molecular and Cellular Biochemistry, Meiji Pharmaceutical University, Tokyo, Japan.
-
- Takaya Akiko
- Department of Natural Products Chemistry, Graduate School of Pharmaceutical Sciences, Chiba University, Chiba, Japan. Medical Mycology Research Center, Chiba University, Chiba, Japan.
-
- Akimitsu Nobuyoshi
- Isotope Science Center, The University of Tokyo, Tokyo, Japan.
この論文をさがす
抄録
<p>Salmonella enterica serovar Typhimurium (Salmonella), a pathogenic bacterium, is a major cause of foodborne diseases worldwide. Salmonella injects multiple virulence factors, called effectors, into cells and causes multiple rearrangements of cellular biological reactions that are important for Salmonella proliferation and virulence. Previously, we reported that Salmonella infection causes loss of MTR4 and RRP6, which are nuclear RNA degradation factors, resulting in the stabilization and accumulation of unstable nuclear RNAs. This accumulation is important for the cellular defense for Salmonella infection. In this study, we examined a series of Salmonella mutant strains, most of which are strains with genes related to effectors translocated by T3SSs encoded on Salmonella pathogenic islands, SPI-1 and SPI-2, that have been depleted. Among 42 Salmonella mutants, 6 mutants' infections canceled loss of MTR4 and RRP6. Proliferation assay of Salmonella in the cell revealed that six mutants showed poor proliferation in the host cell, demonstrating that poor proliferation contributed to cancellation of MTR4 and RRP6 loss. This result indicates that certain events associated with Salmonella proliferation in host cells cause loss of MTR4 and RRP6.</p>
収録刊行物
-
- BioScience Trends
-
BioScience Trends 14 (4), 255-262, 2020-08-31
特定非営利活動法人 バイオ＆ソーシャル・サイエンス推進国際研究交流会