Noise Elimination Electrical Impedance Spectroscopy (neEIS) System for Single Cell Identification
-
- TRAN A.Kiet
- 千葉大学
-
- KAWASHIMA Daisuke
- 千葉大学
-
- SUGARAWA Michiko
- 千葉大学
-
- OBARA Hiromichi
- 東京都立大学
-
- OKEYO Kennedy Omondi
- 京都大学
-
- TAKEI Masahiro
- 千葉大学
抄録
<p>We propose a noise elimination electrical impedance spectroscopy (neEIS) system for single cell identification whose characteristics are cell-free clamp structure and cell-position independent calibration. The cell-position independent calibration consists of three steps; impedance measurement, electrode cell constant calculation, and single cell dielectric properties estimation. The cell-position independent calibration enables elimination the influence of noise associated with ions as well as electrical double layer during the measurement process. We demonstrate an application of neEIS system to discriminate three types of MRC-5 human lung fibroblasts; wild type (WT), GFP-fused histone (HT) modification, and GFP transfected (GFPT) MRC-5 cells suspended in a sucrose medium. Taken together, these results demonstrate that the proposed neEIS system has the potential to improve the performance of biosensors. Thus, this study will bring a new insight into the development of a biosensor system for high accuracy single cell identification as well as inspire the development of a diagnostic device for early fetal lung diseases based on the neEIS system.</p>
収録刊行物
-
- バイオフロンティア講演会講演論文集
-
バイオフロンティア講演会講演論文集 2020.31 (0), 1A14-, 2020
一般社団法人 日本機械学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390570078121737600
-
- NII論文ID
- 130008062142
-
- ISSN
- 24242810
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可