亜硫酸塩が添加され加工されたパパイヤ、トマト、リンゴ、バナナドライフルーツからの DNA の検出可能性について

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  • Detectability of papaya, tomato, apple and banana DNA in dried fruit products processed with food additive sulfites

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Deoxyribonucleic acids (DNAs) in dried fruit products were examined for detectability using real-time polymerase chain reaction (PCR). Endogenous genes with low copy numbers in Carica papaya L. (papaya), Solanum lycopersicum L. (tomato) and <i>Malus domestica</i> (apple) genomic DNAs, i.e., Chymopapain, LAT52 and Apo 5, respectively, were targeted for detection in dried fruit products that were processed with and without food additive sulfites as a bleaching agent, preservative or antioxidant. A total of 13/14 dried papaya, 8/8 dried tomato and 3/3 dried apple products that were processed with sulfites were not detected under a Cq value of 40 in a duplicate real-time PCR test. Despite their undetectability, endogenous 18S rDNA with high copy numbers in the genomic DNA of these fruits was detected at approximately the same amplicon size as the endogenous genes with low copy numbers. Furthermore, BAN, a single-copy endogenous gene found in all dried Musa acuminata (banana) products, was detected using a 50 ng DNA template at a Cq value of 22.33–35.80 regardless of whether the fruit was processed with or without sulfites. Although the dried fruit products that were processed with sulfites may contain DNAs, the yields of extracted and purified DNAs were reduced to the degree that not all endogenous genes could be detected reliably using real-time PCR. This may affect the reliability of real-time PCR testing for detecting specific ingredients in dried fruit products, such as genetically modified fruit and food allergens.

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