Using Microarray Analysis to Evaluate Genetic Polymorphisms Involved in the Metabolism of Environmental Chemicals

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  • Ban Susumu
    Department of Public Health, Hokkaido University, Graduate School of Medicine
  • Kondo Tomoko
    Department of Public Health, Hokkaido University, Graduate School of Medicine
  • Ishizuka Mayumi
    Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University
  • Sasaki Seiko
    Department of Public Health, Hokkaido University, Graduate School of Medicine
  • Konishi Kanae
    Department of Public Health, Hokkaido University, Graduate School of Medicine
  • Washino Noriaki
    Department of Public Health, Hokkaido University, Graduate School of Medicine
  • Fujita Syoichi
    Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University
  • Kishi Reiko
    Department of Public Health, Hokkaido University, Graduate School of Medicine

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Other Title
  • マイクロアレイを用いた環境化学物質代謝に関与する遺伝子多型判定法の開発と問題点

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Abstract

The field of molecular biology currently faces the need for a comprehensive method of evaluating individual differences derived from genetic variation in the form of single nucleotide polymorphisms (SNPs). SNPs in human genes are generally considered to be very useful in determining inherited genetic disorders, susceptibility to certain diseases, and cancer predisposition. Quick and accurate discrimination of SNPs is the key characteristic of technology used in DNA diagnostics. For this study, we first developed a DNA microarray and then evaluated its efficacy by determining the detection ability and validity of this method. Using DNA obtained from 380 pregnant Japanese women, we examined 13 polymorphisms of 9 genes, which are associated with the metabolism of environmental chemical compounds found in high frequency among Japanese populations. The ability to detect CYP1A1 I462V, CYP1B1 L432V, GSTP1 I105V and AhR R554K gene polymorphisms was above 98%, and agreement rates when compared with real time PCR analysis methods (kappa values)showed high validity: 0. 98 (0.96), 0.97 (0.93), 0.90 (0.81), 0.90 (0.91), respectively. While this DNA microarray analysis should prove important as a method for initial screening,it is still necessary that we find better methods for improving the detection of other gene polymorphisms not part of this study.

Journal

  • 福岡醫學雜誌

    福岡醫學雜誌 98 (5), 208-214, 2007-05-25

    Fukuoka Medical Association

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