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Signal Amplification Enzymes for Bioanalysis of Cells and Tissues
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- KANEKO Ryosuke
- Department of Applied Chemistry, Faculty of Engineering, Kyushu University
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- MORI Takeshi
- Department of Applied Chemistry, Faculty of Engineering, Kyushu University Graduate School of Systems Life Sciences, Kyushu University
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- KATAYAMA Yoshiki
- Department of Applied Chemistry, Faculty of Engineering, Kyushu University Graduate School of Systems Life Sciences, Kyushu University Center for Advanced Medical Innovation, Kyushu University Department of Biomedical Engineering, Chung Yuan Christian University
Bibliographic Information
- Other Title
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- 細胞・組織のバイオ分析における増感酵素
- サイボウ ・ ソシキ ノ バイオ ブンセキ ニ オケル ゾウカン コウソ
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Description
<p>In bioanalysis, where proteins in biological samples are labeled with antibodies for detection, enzymatic sensitization reactions are used to enhance the detection signal derived from the dye. In this review, we discussed the desired properties of sensitizing enzymes for bioanalysis and showed that the three conventionally used sensitizing enzymes (horseradish peroxidase, alkaline phosphatase, and β-galactosidase) satisfy these requirements. At the same time, we described the viewpoint calling attention to a lack of conventional enzymes and proposed “mammalian orthogonal enzymes” as a new class of sensitizing enzymes incorporating this viewpoint, and introduced our discovery of α-arabinofuranosidase as an example. If multiple enzymes of this new class are prepared, multi-color detection of multiple antigens will be possible, which is expected to greatly enhance the usefulness of bioanalysis.</p>
Journal
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- BUNSEKI KAGAKU
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BUNSEKI KAGAKU 71 (3), 101-107, 2022-03-05
The Japan Society for Analytical Chemistry