低温電子顕微鏡単粒子解析法による高分解能構造解析

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  • High Resolution Structure Determination by Cryo-Electron Microscopy Single Particle Analysis

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<p>The past decade has seen revolutionary progress in single particle analysis with cryo-electron microscopes. Steady improvement in resolution has culminated in the reports of atomic resolution（1.2 Å）structures of apoferritin in 2020. This review, as a follow-up to my previous review in 2018, summarizes hardware and software improvements that enabled this milestone achievement. A quantitative analysis of the relationship between the number of particles and the resolution is presented and its practical implication in data collection strategies is discussed.</p>

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