Effects of Addition of Blocking Agents on Fluorescence Polarization Immunoassay of Okadaic Acid Using a Microfluidic Device

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  • CHIDA Shunsuke
    Graduate School of Chemical Sciences and Engineering, Hokkaido University
  • TAKAHASHI Kazuki
    Graduate School of Chemical Sciences and Engineering, Hokkaido University
  • FUKUYAMA Mao
    Institute of Multidisciplinary Research for Advanced Materials, Tohoku University
  • KASUYA Motohiro
    Faculty of Production Systems Engineering and Sciences, Komatsu University
  • MAEKI Masatoshi
    Division of Applied Chemistry, Faculty of Engineering, Hokkaido University
  • ISHIDA Akihiko
    Division of Applied Chemistry, Faculty of Engineering, Hokkaido University
  • TANI Hirofumi
    Division of Applied Chemistry, Faculty of Engineering, Hokkaido University
  • SHIGEMURA Koji
    Tianma Japan, Ltd.
  • ZHERDEV Anatoly V.
    A. N. Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences
  • EREMIN Sergei A.
    A. N. Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences Department of Chemical Enzymology, Faculty of Chemistry, M. V. Lomonosov Moscow State University
  • HIBARA Akihide
    Institute of Multidisciplinary Research for Advanced Materials, Tohoku University
  • TOKESHI Manabu
    Division of Applied Chemistry, Faculty of Engineering, Hokkaido University

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Other Title
  • マイクロ流体デバイスを利用した下痢性貝毒オカダ酸の蛍光偏光免疫分析におけるブロッキング剤添加の影響
  • マイクロ リュウタイ デバイス オ リヨウ シタ ゲリセイ カイドク オカダサン ノ ケイコウ ヘンコウ メンエキ ブンセキ ニ オケル ブロッキングザイ テンカ ノ エイキョウ

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Abstract

<p>In analysis using microfluidic devices, adsorption of samples and other substances on the channel walls can be a problem. Especially in immunoassays using polydimethylsiloxane (PDMS) devices, the adsorption of antibodies and other substances to be measured greatly affects measurement performance. In this study, we investigated the effect of the addition of two blocking agents on the detection sensitivity of okadaic acid (OA), a diarrheal shellfish poison, by fluorescence polarization immunoassay. In addition, the dissociation constants for the antibody and tracer were calculated from the obtained calibration curve.</p>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 72 (3), 133-138, 2023-03-05

    The Japan Society for Analytical Chemistry

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