Cellular characterization of hiPS CMs cultured on PMEA analogous polymers with different bound water content

DOI IR (HANDLE) Open Access
  • KURITA Hiroko
    Department of Molecular and Material Sciences, Interdisciplinary Graduate School of Engineering Sciences, Kyushu University : Graduate Student
  • KOBAYASHI Shingo
    Institute for Materials Chemistry and Engineering, Kyushu University
  • ANADA Takahisa
    Institute for Materials Chemistry and Engineering, Kyushu University Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
  • TODO Mitsugu
    Research Institute for Applied Mechanics, Kyushu University Department of Molecular and Material Sciences, Interdisciplinary Graduate School of Engineering Sciences, Kyushu University
  • TANAKA Masaru
    Institute for Materials Chemistry and Engineering, Kyushu University Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

Bibliographic Information

Published
2023-09
Resource Type
departmental bulletin paper
DOI
  • 10.15017/6786935
Publisher
Interdisciplinary Graduate School of Engineering Sciences, Kyushu University

Search this article

Description

In recent years, human iPS cell-derived cardiomyocytes (hiPS-CMs) have attracted much attention in the arena of regenerative therapies. However, hiPS-CMs are known to be immature and have lower contractility than the adult heart. In this study, we investigated the effect of material properties of scaffold surfaces on hiPS-CMs with the aim of improving the contractility of hiPS-CMs. PMEA analogs with varying surface-bound water content, which affect cell adhesion/proliferation behavior, were selected as scaffold materials to coat PET substrates. The cell characteristics of the hiPS-CMs seeded on the scaffolds were evaluated. The initial cell adhesion area tended to be smaller on substrates with a higher amount of bound water. With further incubation, the hiPS-CMs formed self-pulsating aggregates on the substrate. On the substrates with high bound water content, the area of the hiPS-CMs aggregates tended to be smaller while the pulsation behavior became greater. The results indicate that PMEA analogues might affect the cellular properties of hiPS-CMs.

Journal

Keywords

Details 詳細情報について

  • CRID
    1390577431256360960
  • NII Book ID
    AA1147319X
  • DOI
    10.15017/6786935
  • HANDLE
    2324/6786935
  • ISSN
    13467883
  • Text Lang
    en
  • Article Type
    departmental bulletin paper
  • Data Source
    • JaLC
    • IRDB
    • OpenAIRE
  • Abstract License Flag
    Allowed

Report a problem

Back to top