Cellular characterization of hiPS CMs cultured on PMEA analogous polymers with different bound water content

DOI IR (HANDLE) Open Access
  • KURITA Hiroko
    Department of Molecular and Material Sciences, Interdisciplinary Graduate School of Engineering Sciences, Kyushu University : Graduate Student
  • KOBAYASHI Shingo
    Institute for Materials Chemistry and Engineering, Kyushu University
  • ANADA Takahisa
    Institute for Materials Chemistry and Engineering, Kyushu University Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
  • TANAKA Masaru
    Institute for Materials Chemistry and Engineering, Kyushu University Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
  • TODO Mitsugu
    Research Institute for Applied Mechanics, Kyushu University Department of Molecular and Material Sciences, Interdisciplinary Graduate School of Engineering Sciences, Kyushu University

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In recent years, human iPS cell-derived cardiomyocytes (hiPS-CMs) have attracted much attention in the arena of regenerative therapies. However, hiPS-CMs are known to be immature and have lower contractility than the adult heart. In this study, we investigated the effect of material properties of scaffold surfaces on hiPS-CMs with the aim of improving the contractility of hiPS-CMs. PMEA analogs with varying surface-bound water content, which affect cell adhesion/proliferation behavior, were selected as scaffold materials to coat PET substrates. The cell characteristics of the hiPS-CMs seeded on the scaffolds were evaluated. The initial cell adhesion area tended to be smaller on substrates with a higher amount of bound water. With further incubation, the hiPS-CMs formed self-pulsating aggregates on the substrate. On the substrates with high bound water content, the area of the hiPS-CMs aggregates tended to be smaller while the pulsation behavior became greater. The results indicate that PMEA analogues might affect the cellular properties of hiPS-CMs.

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