Comparison of accuracy of Flow Cytometric Crossmatch between two hospitals

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  • フローサイトメトリークロスマッチにおける2施設間の比較検討
  • フローサイトメトリークロスマッチ ニ オケル 2 シセツ カン ノ ヒカク ケントウ

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Abstract

<p>Background: Flow Cytometric Crossmatch (FCXM) is widely used as a histocompatibility test before renal transplantation. However, because the procedure and criteria for FCXM are not standardized, the quality control of FCXM is insufficient. Patients and Methods: We compared the FCXM results of 44 preoperative renal transplantation cases performed at our hospital and the Fukuoka Red Cross Hospital to validate the accuracy of FCXM at our hospital and investigate the causes of discrepancies between the results of the two hospitals. Results: The T-cell positive and negative concordance rates were both 100% (44/44) at our institution, whereas the B-cell positive and negative concordance rates were 100% (7/7) and 86.5% (32/37), respectively, as determined on the basis of data from the Fukuoka Red Cross Hospital. Two of the five patients with discrepancies had donor-specific antibodies (DSAs), two had non-DSAs, and one had negative anti-human leukocyte antigen antibodies. In the DSA-negative 3 cases, the lack of pronase treatment may have led to a false-positive result. Conclusion: Even though the concordance rate between FCXMs at both centers was relatively good when a weakly positive B-cell result is obtained in an FCXM without pronase treatment, the possibility of false-positive results should be considered, and the results of anti-human leukocyte antigen antibody identification tests should also be evaluated.</p>

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