Trigonelline Attenuates Lipopolysaccharide-Induced Nitric Oxide Production and Downregulates Inflammation Associated Gene Expressions

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  • AIZAWA Yumi
    Faculty of Applied Bioscience, Tokyo University of Agriculture
  • SUZUKI Tsukasa
    Faculty of Applied Bioscience, Tokyo University of Agriculture
  • SAITO Akihiro
    Faculty of Applied Bioscience, Tokyo University of Agriculture
  • SATOH Kiyotoshi
    Institute for Advanced Biosciences, Keio University
  • OGURA Tatsuki
    Institute for Advanced Biosciences, Keio University
  • WAKAYAMA Masataka
    Institute for Advanced Biosciences, Keio University
  • SOGA Tomoyoshi
    Institute for Advanced Biosciences, Keio University
  • TSUJII Yoshimasa
    Faculty of Applied Bioscience, Tokyo University of Agriculture
  • HOMMA Kazuhiro
    Faculty of Applied Bioscience, Department of Nutritional Science, Tokyo University of Agriculture
  • INOUE Hirofumi
    Faculty of Applied Bioscience, Department of Nutritional Science and Food Safety, Tokyo University of Agriculture

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Other Title
  • リポポリサッカライドによる一酸化窒素および炎症関連分子群に及ぼすトリゴネリンの作用機序

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Abstract

<p>　Trigonelline, an N-methylnicotinic acid, is found in fenugreek (Trigonella foenum-graecum L.) and coffee beans. Trigonelline has been reported to prevent cardiovascular and Alzheimer's diseases. However, the potential pharmacological application of trigonelline in in fl ammatory responses is poorly understood, particularly in macrophages. In the present study, we investigated the effects and underlying molecular mechanisms of trigonelline on 100ng/mℓ lipopolysaccharide (LPS) -induced inflammatory responses in RAW264.7 mouse macrophages. 50μM Trigonelline elicited a marked reduction in LPS-mediated production of nitric oxide (NO) compared with LPS-treated cells. Subsequently, the mRNA expression levels of inducible nitric oxide (iNOS), cyclooxygenase-2 (COX2), and pro-inflammatory cytokines, including tumor necrosis factor α (TNF-α), interleukin (IL) -6, and IL-1β in LPS-treated cells were significantly decreased by trigonelline co-treatment. Furthermore, we found that treatment of cells with LPS elevated the gene expression of reactive oxidative species (ROS) -associated molecules, such as p47phox and p67phox, whereas trigonelline treatment significantly decreased them. Our data suggested that trigonelline inhibited inflammation and oxidative stress, resulting in the potential application of this compound in the prevention of infl ammatory diseases.</p>

Journal

  • Food Preservation Science

    Food Preservation Science 46 (6), 275-280, 2020

    Japan Association of Food Preservation Scientists

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