書誌事項
- タイトル別名
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- A Simple Spectrophotometric Assay for Determination of Selenium Concentration in the Hospital Preparation
抄録
<p>A simple spectrophotometric assay was developed for quantitative determination of selenium in selenium injection which is a hospital preparation for patients with selenium deficiency. Aliquots of the samples (500 µL) added 20 µL of 2.5 M formic acid and 20 µL of 18.5 mM O-phenylene diamine were incubated for two hours at room temperature. The absorbance was measured in the mixture at 333 nm by using NanoDrop®, a convenient instrument for spectrophotometric assay. The standard curve for determining selenium concentration was linear in the range of 1.25-30 µg/mL (r > 0.999). Coefficients of variation for intra- and inter-day assays were 0.3 to 5.5% and 0.8 to 2.8%, respectively. Assay validation was confirmed by five independent measurements of 50 µg/mL selenium injection in three pharmacists with the accuracy of 0.0 to 1.6%. This assay method was successfully applied to the quantitative determination of selenium in selenium injection.</p>
収録刊行物
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- 医療薬学
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医療薬学 44 (11), 582-586, 2018-11-10
一般社団法人日本医療薬学会
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詳細情報
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- CRID
- 1390845702321710592
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- NII論文ID
- 130007743078
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- ISSN
- 18821499
- 1346342X
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可