CONSTRUCTION OF A HIGHLY ACCURATE PREDICTION FORMULA FOR CD34<sup>+</sup> CELL YIELD IN PERIPHERAL BLOOD HEMATOPOIETIC STEM CELL HARVEST

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  • Higuchi Hiroji
    Division of Transfusion Medicine and Cell Therapy, University of Yamanashi Hospital
  • Takano Katsuhiro
    Division of Transfusion Medicine and Cell Therapy, University of Yamanashi Hospital Department of Clinical Laboratory, University of Yamanashi Hospital
  • Yamanaka Hiroyo
    Division of Nursing, University of Yamanashi Hospital
  • Sakamoto Mihoko
    Department of Clinical Laboratory, University of Yamanashi Hospital
  • Endo Masumi
    Department of Clinical Laboratory, University of Yamanashi Hospital
  • Kaneko Makoto
    Department of Laboratory Medicine, Tokyo Medical University
  • Kirito Keita
    Department of Hematology and Oncology, University of Yamanashi
  • Suzuki-Inoue Katsue
    Division of Transfusion Medicine and Cell Therapy, University of Yamanashi Hospital Department of Clinical Laboratory, University of Yamanashi Hospital

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  • 精度の高い末梢血幹細胞採取用収量予測計算式の構築

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<p>The conventional prediction formula for CD34+ cell yield in peripheral blood hematopoietic stem cell harvest (PBSCH) is composed of peripheral blood CD34+ cell number, the processed blood volume, and body weight. In the present study, we proposed a novel prediction formula that includes the collection efficiency. The collection efficiency is defined by the following: the number of collected cells/the total number of CD34+cells in the processed blood. We evaluated the correlation between several factors that may affect the actual collection efficiency and the collection efficiency calculated using the above formula and the actual CD34+ cell number obtained by PBSCH. Among these, 32.5× (WBC/Hct) -0.364 showed the best correlation with collection efficiency. Therefore, we included it to represent collection efficiency in the prediction formula. These findings indicate that high WBC numbers leads to low CD34+ cell yield, presumably because the WBC sedimentation rate is low in the presence of high WBC numbers. Based on analysis of 48 PBSCH from 23 adult patients, the new prediction formula containing the collection efficiency showed higher prediction accuracy than the conventional prediction formula. This prediction formula allows prediction of the number of CD34+ cells before PBSCH and identification of the best conditions for PBSCH.</p>

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