Determination of Antifungal Drugs in Fish and Livestock Products Using LC-MS/MS
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- Nakagawa Yukiko
- Tokyo Metropolitan Institute of Public Health
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- Kanda Maki
- Tokyo Metropolitan Institute of Public Health
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- Hayashi Hiroshi
- Tokyo Metropolitan Institute of Public Health
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- Matsushima Yoko
- Tokyo Metropolitan Institute of Public Health
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- Ohba Yumi
- Tokyo Metropolitan Institute of Public Health
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- Koike Hiroshi
- Tokyo Metropolitan Institute of Public Health
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- Nagano Chieko
- Tokyo Metropolitan Institute of Public Health
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- Sekimura Kotaro
- Tokyo Metropolitan Institute of Public Health
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- Otsuka Kenji
- Tokyo Metropolitan Institute of Public Health
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- Sasamoto Takeo
- Tokyo Metropolitan Institute of Public Health
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- Hashimoto Tsuneo
- Tokyo Metropolitan Institute of Public Health
Bibliographic Information
- Other Title
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- LC-MS/MSによる畜水産食品中抗真菌薬の一斉分析法
- LC-MS/MS ニ ヨル チクスイサン ショクヒン チュウ コウシンキンヤク ノ イッセイ ブンセキホウ
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Abstract
<p>We developed an analytical method for determining 15 antifungal drugs, 2 antiparasitic drugs, and 3 veterinary drugs in fish and livestock products using LC-MS/MS. First, 50% ethanol was added to their products, and the mixture was homogenized to reduce drug degradation. Thereafter, 20 drugs were extracted from the pretreated sample mixture using acetonitrile. Cleanup was performed using an alumina-N SPE cartridge. Finally, chromatographic separation was performed using a fully porous octadecyl silanized silica column. The new method is applicable to fish in which the matrix hampers accurate analysis. It was validated on 8 fish and livestock products. Drug recovery rates ranged from 70.2 to 109.3%, RSDs of repeatability were <18.0%, and RSDs of within-laboratory reproducibility were <18.7%. It fulfills the Japanese guideline criteria. The limits of quantification were estimated as 3 ng/g.</p>
Journal
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- Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
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Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 60 (3), 52-60, 2019-06-25
Japanese Society for Food Hygiene and Safety
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Keywords
Details 詳細情報について
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- CRID
- 1390845713086597632
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- NII Article ID
- 130007688189
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- NII Book ID
- AN00117741
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- ISSN
- 18821006
- 00156426
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- NDL BIB ID
- 029861941
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- PubMed
- 31391411
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed