プリン・ピリミジンヌクレオシドの過酸化水素によるチミジン取り込みへの効果

DOI Web Site オープンアクセス
  • 田中 康一
    兵庫医療大・薬・薬理 兵庫医科大学薬理学講座 鹿児島大学大学院医歯学総合研究科生体機能制御学講座歯科応用薬理学分野
  • 富田 和男
    兵庫医療大・薬・薬理 鹿児島大学大学院医歯学総合研究科生体機能制御学講座歯科応用薬理学分野
  • 北中 順惠
    兵庫医科大学薬理学講座
  • 北中 純一
    兵庫医科大学薬理学講座
  • 佐藤 友昭
    鹿児島大学大学院医歯学総合研究科生体機能制御学講座歯科応用薬理学分野
  • 竹村 基彦
    兵庫医科大学薬理学講座
  • 西山 信好
    兵庫医療大・薬・薬理

書誌事項

タイトル別名
  • Effects of purine and pyrimidine nucleosides on hydrogen peroxide-induced thymidine incorporation

説明

<p>We have found that cultured differentiated astrocytes pretreated with N6, 2'-O-dibutyryladenosine 3',5'-cyclic monophosphate (DBcAMP), a permeable analogue of cAMP, incorporate thymidine, but not uridine, via specific nucleoside transporters, ENT2 and CNT3, into TCA insoluble fraction for repair on DNA injury in the presence of hydrogen peroxide (H2O2) at an early time, and these phenomena are specific in differentiated astrocytes, but not undifferentiated astrocytes and neurons. Purine and pyrimidine nucleosides are DNA and RNA precursors consisting of base and ribose.</p><p>We studied the influence of purine and pyrimidine nucleosides on H2O2-induced thymidine incorporation into cultured astrocytes. Adenosine and guanosine, RNA precursor purine nucleosides, decreased H2O2-induced thymidine incorporation. On the other hand, Deoxyadnosine and deoxyguanosine, DNA precursor purine nucleosides, increased it. Cytidine, RNA precursor pyrimidine nucleoside, did not influence it, but deoxycitidine, DNA precursor pyrimidine nucleoside, decreased it.</p><p>These results indicate that H2O2-induced thymidine incorporation into cultured astrocytes are increased synergistically by purine nucleosides, but not pyrimidine nucleosides.</p>

収録刊行物

詳細情報 詳細情報について

問題の指摘

ページトップへ