各種骨補填材におけるマクロファージ・骨芽細胞分化能およびBone Morphogenetic Protein-2の担体としての有用性に関する<i>in vitro</i>評価

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  • 小林 真左子
    ベルン大学医学部頭蓋顎顔面外科学講座
  • 河野 通秀
    ベルン大学医学部頭蓋顎顔面外科学講座 東京医科大学口腔外科学分野
  • 永井 孝宏
    ベルン大学医学部頭蓋顎顔面外科学講座 新潟大学大学院医歯学総合研究科顎顔面口腔外科学分野
  • 片桐 浩樹
    ベルン大学医学部頭蓋顎顔面外科学講座 日本歯科大学新潟生命歯学部先端研究センター
  • 飯塚 建行
    ベルン大学医学部頭蓋顎顔面外科学講座

書誌事項

タイトル別名
  • <i>In vitro</i> evaluation of bone grafting materials on macrophage polarization, osteogenic differentiation, and the potential as bone morphogenetic protein-2 carriers
  • 各種骨補填材におけるマクロファージ・骨芽細胞分化能およびBone Morphogenetic Protein-2の担体としての有用性に関するin vitro評価
  • カクシュ ホネ ホテンザイ ニ オケル マクロファージ ・ コツガ サイボウ ブンカノウ オヨビ Bone Morphogenetic Protein-2 ノ タンタイ ト シテ ノ ユウヨウセイ ニ カンスル in vitro ヒョウカ

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<p>Various bone grafting materials are used for bone augmentation procedures in the field of oral-maxillofacial surgery. Knowledge of biomaterial characteristics is crucial in order to choose the appropriate materials for each clinical case. The purpose of the present study was to investigate not only the conventional method of comparison of osteogenic potential of different bone grafting materials, but also evaluate the macrophage behavior and the potential as carriers of growth factors in vitro.</p><p>　Cell attachment/proliferation on bone grafting materials and the gene expressions of cell differentiation markers were investigated in the human leukemia monocytic cell line THP-1-derived macrophages and mouse stromal ST-2 cells, when cultured on the three different grafting materials of 1) demineralized freeze-dried bone allografts (DFDBA)(DBX®, Synthes Inc. USA), 2) deproteinized bovine bone mineral (DBBM)(Bio-Oss®, Geistlich Pharma AG, Switzerland), and 3) biphasic calcium phosphate (BCP)(Maxresorb® granules, Botiss AG, Germany). Furthermore, bone morphogenetic protein-2 (BMP-2) adsorption, release kinetics, and osteoblastic activities of BMP-2-loaded materials were evaluated.</p><p>　Higher cell attachment/proliferation potentials in both THP-1-derived macrophages and ST-2 cells were observed when cultured on BCP. Interestingly, DFDBA showed high TNF-α expression with a strong tendency toward M1 phenotypes, whereas BCP showed high IL-10 expression in macrophages and was polarized toward M2 phenotypes. Furthermore, DFDBA significantly increased mRNA levels of bone sialoprotein (BSP) when compared to the other materials in ST-2 cells. DBBM adsorbed a higher amount of BMP-2 when compared with the other materials while the DFDBA released BMP-2 faster. DFDBA showed higher osteopromotive potential including higher mRNA levels of ALP and BSP when used in combination with BMP-2, compared to BMP-2 alone.</p><p>　From this limited study, BCP showed superior cytocompatibility. Nevertheless, DFDBA might be a good option when focusing on higher osteopromotive potential, and for use as a BMP-2 carrier.</p>

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