Live Single-cell質量分析法による動物細胞内代謝物局在解析

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  • Live Single-cell mass spectrometry for organelle metabolomics in animal cells
  • Live Single-cell シツリョウ ブンセキホウ ニ ヨル ドウブツ サイボウ ナイ タイシャブツキョクザイカイセキ

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<p>We have developed “Live Single-cell Mass Spectrometry” technique for a direct analysis of endogenous compounds in a single living cell.　The technique comprises a sampling from a living animal cell captured under video-microscopic observation, a nano-electrospray ionization of the sampled cell or cytosols and a high-resolution mass spectrometry.　The technique with stable isotope-labeled reagents realizes quantitatively to trace the metabolic processes of endogenous compounds as well as xenobiotics in a live cell.　In this paper, we demonstrate localization analysis of histidine metabolites in single living RBL-2H3 cells and to trace histidine metabolism using stable isotope labeling.　This method has enabled us to analyze not only location of metabolic pathways but also metabolic processes of interest molecules, and it can be used in various organelle metabolomics in a single live cell.　Currently, the technique is further improved and evolved to be a robotic cell manipulator with a confocal bio-imaging system so that a single cell or cytosol from a single live cell is automatically sampled based on a captured 3D bioimage.</p>

収録刊行物

  • 植物の生長調節

    植物の生長調節 55 (2), 92-99, 2020

    一般社団法人 植物化学調節学会

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