An Efficient Protocol of Protocorm–Like Bodies Regeneration from Callus Cultures of Gastrodia elata Blume and the Further Associations with Mycorrhizal Fungi
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- Yeh Chih-Hsin
- Institute of Tropical Agriculture, Faculty of Agriculture, Kyushu University | Taoyuan District Agricultural Research and Extension Station, Council of Agriculture
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- Liao Fang-Shin
- Institute of Tropical Agriculture, Faculty of Agriculture, Kyushu University | Taoyuan District Agricultural Research and Extension Station, Council of Agriculture
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- Huang Kuang-Liang
- Institute of Tropical Agriculture, Faculty of Agriculture, Kyushu University | Department of Horticultural Science, National Chiayi University
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- 宮島 郁夫
- 九州大学熱帯農学研究センター
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- Lee Yung-I
- Institute of Tropical Agriculture, Faculty of Agriculture, Kyushu University | Biology Department, National Museum of Natural Science | Department of Life Sciences, National Chung Hsing University
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説明
In this study, we reported a reliable protocol of PLBs regeneration from embryogenic callus cultures of Gastrodia elata, a fully mycoheterotrophic orchid and an endangered herbal medicinal plant. The continuous immersion cultures with TDZ (0.5 and 1 mg · L^〈–1〉) and BA (10 mg · L^〈–1〉) improved PLBs regeneration frequency and rate. Histological studies indicated that starch grains were abundant within in the cytoplasm of embryogenic callus and developing PLBs. The formation of PLBs from cell aggregates in the periphery region of embryogenic callus was supposed to be the multicellular origin pathway. After inoculated with suitable Armillaria isolates, PLBs enlarged further and resulted in the formation of tubers. The results of phylogenetic analysis and inoculation experiments indicated that the asymbiotic PLBs could establish the appropriate association with Armillaria mellea complex, including A. mellea and A. gallica. Thus, this protocol of PLBs regeneration from callus cultures is beneficial for the multiplication of selected elites, and could be used as an experimental system for the molecular investigations of the mycoheterotrophic orchid – mycorrhizal fungi interactions.
収録刊行物
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- Journal of the Faculty of Agriculture, Kyushu University
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Journal of the Faculty of Agriculture, Kyushu University 62 (1), 39-46, 2017-02-24
九州大学大学院農学研究院
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詳細情報 詳細情報について
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- CRID
- 1390853649614854144
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- NII論文ID
- 120005981101
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- NII書誌ID
- AA00247166
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- DOI
- 10.5109/1798139
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- HANDLE
- 2324/1798139
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- ISSN
- 00236152
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- Crossref
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