Preparation of Anti-fragrant Monoclonal Antibodies by the Rat Lymph Node Method and Their Characterization Using Enzyme-linked Immunosorbent Assay

DOI HANDLE 被引用文献1件 オープンアクセス
  • Ohashi Shintaro
    Laboratory of Food Analysis, Division of Food Biotechnology, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
  • Nagatomo Kazutaka
    Laboratory of Food Analysis, Division of Food Biotechnology, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
  • Miyamoto Koichiro
    Program of Food Science and Technology, Course of Applied Biological Science, Department of Bioresource and Bioenvironment, School of Agriculture, Kyushu University
  • 都甲 潔
    九州大学大学院システム情報科学研究院
  • 三浦 則雄
    九州大学産学連携センター
  • 松本 清
    九州大学大学院農学研究院生物資源環境科学府生物機能科学専攻食品バイオ工学講座食品分析学分野

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In the food and beverage industries there is a great demand for a sensitive and easy detection system of fragrant compounds in order to ensure product quality control and to avoid contamination. Benzaldehyde (BZ) and furfural (FF) are typical fragrant compounds for peach?flavored beverages, and methyl anthranilate (MA) is a typical flavor of grape beverages. For sensitive detection of these flavors by immunoreaction, we prepared monoclonal antibodies against BZ, FF and MA by using the rat lymph node method. The antibody for BZ had high selectivity toward BZ compared with other fragrant compounds and high affinity with an affinity constant of 1.9×106M?1. The limit of detection (LOD) of BZ by indirect competitive ELISA (ic?ELISA) was 0.6 ng mL?1. For FF, anti?FF mAb had high selectivity toward FF and high affinity constant of 2.5×104 M?1. The LOD of FF by ic?ELISA was 100 ng mL?1. For MA, anti?MA mAb had high selectivity toward MA and high affinity constant of 1.3×105M?1. The LOD of MA by ic?ELISA was 200 ng mL?1. These monoclonal antibodies were suitable for detection of very small amounts of fragrant compounds in rinsed water of beverage industries.

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詳細情報 詳細情報について

  • CRID
    1390853649614866048
  • NII論文ID
    120002223682
  • NII書誌ID
    AA00247166
  • DOI
    10.5109/17808
  • HANDLE
    2324/17808
  • ISSN
    00236152
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • IRDB
    • Crossref
    • CiNii Articles

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