Establishment of Pollen Storage Method for Pointed Gourd (Trichosanthes dioica Roxb.) Originating in South Asia

  • HASSAN Jahidul
    Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
  • HOSSAIN M. Mofazzal
    Department of Horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University
  • MIYAJIMA Ikuo
    Institute of Tropical Agriculture, Kyushu University

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Other Title
  • 南アジア原産の食用カラスウリ(Trichosanthes dioica Roxb.) における花粉貯蔵法の確立

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Description

As pointed gourd (Trichosanthes dioica Roxb.), which is dioecious and originates from South Asia, has ephemeral flowers (one day flowering), asynchrony of anthesis between male and female flowers is a major concern for fruits production. Properly stored pollen might therefore be helpful to overcome pollination problems. Pollen viability and pollen germination of this species was investigated at anthesis, and after short-term and long-term storage under different temperature conditions. Pollen stored at -20 performed well with regard to pollen viability and germination for the maximum duration when compared to room temperature and 4℃. More than 20% of pollen grains induced germination after 3 days of storage at room temperature, after 1 week at 4℃ and after 1 month at -20℃. Viable stored pollens after 1 month at 4℃ and after 4 months at -20℃ yielded fruit at 26. 7% by hand pollination in an open field. However, a fruit set rate of over 80% was confirmed using pollens after 3 days stored at 4℃ and after 2 weeks stored at -20℃.

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