Mitochondrial nucleoid morphology and respiratory function are altered in Drp1-deficient HeLa cells
Bibliographic Information
- Other Title
-
- JB Special Issue : Dynamics Regulation of Mitochondria
Search this article
Abstract
<jats:title>Abstract</jats:title> <jats:p>Mitochondria are dynamic organelles that frequently divide and fuse with each other. The dynamin-related GTPase protein Drp1 has a key role in mitochondrial fission. To analyse the physiological roles of Drp1 in cultured human cells, we analysed Drp1-deficient HeLa cells established by genome editing using CRISPR/Cas9. Under fluorescent microscopy, not only mitochondria were elongated but their DNA (mtDNA) nucleoids were extremely enlarged in bulb-like mitochondrial structures (‘mito-bulbs’) in the Drp1-deficient HeLa cells. We further found that respiratory activity, as measured by oxygen consumption rates, was severely repressed in Drp1-deficient HeLa cells and that this was reversible by the co-repression of mitochondrial fusion factors. Although mtDNA copy number was not affected, several respiratory subunits were repressed in Drp1-deficient HeLa cells. These results suggest that mitochondrial fission is required for the maintenance of active respiratory activity and the morphology of mtDNA nucleoids in human cells.</jats:p>
Journal
-
- The journal of biochemistry
-
The journal of biochemistry 167 (3), 287-294, 2020-03
Tokyo : Japanese Biochemical Society
- Tweet
Details 詳細情報について
-
- CRID
- 1524232504525733632
-
- NII Article ID
- 40022185554
-
- NII Book ID
- AA00694073
-
- ISSN
- 0021924X
- 17562651
-
- NDL BIB ID
- 030308181
-
- Text Lang
- en
-
- NDL Source Classification
-
- ZR2(科学技術--生物学--生化学)
-
- Data Source
-
- NDL
- Crossref
- CiNii Articles
- KAKEN